Porcine Claudin 4 (CLDN4) ELISA Kit from MyBioSource.com

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Porcine Claudin 4 (CLDN4) ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Claudin 3 (also known as CLDN3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine CLDN3 antibody. CLDN3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine CLDN3 Antibody is added and binds to CLDN3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CLDN3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine CLDN3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm